Figure 6.

Resampling method to determine target sequencing depth. A resampling analysis was conducted to determine the number of total fragment reads needed to achieve desirable levels of coverage. Plotted is how the number of uniquely identifiable sequenced DNA fragments resulting from sheared ApeKI fragments varies with the total number of sequenced DNA fragments. Results were generated based on empirically determined frequencies of fragment reads from ∼8.69 Gb of B73 DNA sequence reads. The red, blue, and green points highlight the number of total fragment reads necessary to observe 90%, 80%, and 70% of the potential fragments, respectively.