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. 2013 Apr;193(4):1095–1105. doi: 10.1534/genetics.112.149120

Figure 3.

Figure 3

Telomere length positively correlates with percentage of intracellular dGTP. (A) dNTP concentrations in a wild-type strain and the rnr1 mutants were measured. Mean ± SE is shown for each strain. Data for the wild type, rnr1-Q288A, rnr1-R293A, and rnr1-Y285A strains were previous reported (Kumar et al. 2010). (B) Strains of the indicated genotypes were assayed for telomere length by telomere VI-R PCR after being passaged for at least 100 generations (a representative gel is shown). The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for at least three independent isolates. (C) Strains of the indicated genotype were plotted for telomere length vs. dGTP as a fraction of total dNTP levels. Each point indicates the mean for each of these values, and error bars indicate the standard error. (D) Strains of the indicated genotypes, generated from the sporulation of rnr1/RNR1 tlc1Δ/TLC1 diploids, were assayed for telomere length by Y′ telomere PCR after being passaged for ∼30 generations. The change in telomere length, compared to wild-type telomere length, was quantified and plotted. Mean ± SE is shown for three independent isolates.