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. 2013 Apr;193(4):1117–1133. doi: 10.1534/genetics.113.149849

Figure 7.

Figure 7

GCR frequency in 0.003% MMS. (A) GCR frequency for tel1-Δ interactions. Strains were grown in YEPD + 0.003% MMS for 15 hr and subsequently plated onto C-Arg-Ser + canavanine + 5-FOA to simultaneously select for the loss of CAN1 and URA3 markers on the left end of chromosome V. Error bars represent standard deviations from two independent cultures per strain, each plated two times. The rad27-Δ mutants are plotted separately due to scale. (B) GCR frequency in 0.003% MMS with or without the CDC13-EST2 fusion plasmid. The indicated strains containing either an empty vector (black bars) or the CDC13-EST2 fusion (gray bars) were grown in YEPD + 0.003% MMS for 15 hr and subsequently plated onto C-Arg-Ser + canavanine + 5-FOA to select for simultaneous loss of CAN1 and URA3 markers. Error bars represent standard deviations from two independent cultures per strain, each plated two times.