Figure 4.

(A and B) Ectopically introduced matA transgene induces gene silencing at both resident and ectopic matA. (A) Schematic representation of the removal of the ectopic matA transgene using 5′-FOA selection. (B) Expression analysis of the matA gene in a strain having duplication (before FOA) and a strain that was recovered upon excision of the ectopic matA (after 5′-FOA). Transcript levels were analyzed in undifferentiated hyphae (H) and in reproductive tissue 6 days PI of sexual development (R6).