Figure 1.

Systematic overexpression screen to identify genes capable of inducing filamentous growth. (A) Overview of the experimental design. Sample set of overexpression constructs from plate 39 (of 72 plates in total) is shown. Spotted cultures were assayed for invasive growth in an arrayed pattern with 48 spots per plate. Cultures were rearrayed in an altered pattern during retesting to control for positional effects. (B) Sampling of assay results for the wild-type background strain and eight overexpression mutants yielding invasive growth under conditions of nitrogen sufficiency. The degree of invasive growth was quantified by determining the pixel intensity of the spotted culture postwash relative to its prewash intensity. Complete listing of quantified scores for the genes identified in this screen is provided in Figure S3.