Figure 3. PS-conjugated Ags were captured by professional APCs effectively.

(A) Splenocytes were classified into five subpopulations (I to V) based on the expression pattern of CD11b and CD11c. I:CD11b⁻CD11c⁻ cells, II:CD11b^intCD11c⁻ cells, III:CD11b^highCD11c⁻ cells, IV:CD11b⁺CD11c⁺ cells, V:CD11b⁻CD11c⁺ cells. (B, C) Each isolated population was co-cultured with sfGFP, sfGFP-PS or sfGFP-liposome for 60 min, and then the amount of uptake was analyzed by flow cytometry. (D, E) Each population of isolated cells was co-cultured with DQ-OVA or DQ-OVA-PS for 60 min, and then the efficiency of antigen degradation processing was analyzed by flow cytometry.