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. 2013 Mar 22;8(3):e59509. doi: 10.1371/journal.pone.0059509

Figure 7. L. donovani infection increases apoptosis of erythroblasts.

Figure 7

Apoptosis of bone marrow erythroblasts was examined by flow cytometry from control non-infected hamsters and L. donovani infected hamsters. Apoptosis was determined by Annexin-V binding (A) and the TUNEL Assay (B). Representative flow cytometry plots are from a control non-infected hamster and a hamster infected with L. donovani for 7 weeks. (A) Bone marrow cells were labeled with anti- E-cadherin antibody-e-Fluro 660 and FITC-Annexin V. Dead cells were excluded with 7-amino actinomycin (7-ADD). (B) BrdUTP incorporation by the TUNEL technique in erythroblasts was detected using Alexa-488 anti-BrdUTP and anti-E-cadherin-e-Fluro 660. (C) Percentage of E-cadherin positive erythroblasts that are Annexin-V positive. (D) Percentage of E-cadherin positive erythroblasts that are TUNEL positive. Data represent mean +/− SEM of 4 hamsters in each group. *p<0.05, Student’s t test.