Skip to main content
. 2013 Mar 22;8(3):e59563. doi: 10.1371/journal.pone.0059563

Figure 6. miR-34a inhibits migration and invasion.

Figure 6

(a) PM-34a inhibits migration in vitro. Rko and HCT-116 cells were transfected with a constitutively active Src expression construct (A-Src), empty vector (Vector), PM-34a or negative control (NC) as indicated. After 48 h, the cells were counted and equal numbers of cells were seeded on top of transwell plates with serum free medium and 0.1% BSA. After 14 h, the migrated cells were measured as described in Materials and Methods. Data are represented as the percentage of migrated cells, as mean±s.d. of triplicates (*p≤0.05). (b) PM-34a inhibits invasion in vitro. Rko and HCT-116 cells were transfected with a constitutively active Src expression construct (A-Src), empty vector (Vector), PM-34a or negative control (NC) as indicated. After 48 h, the cells were counted and equal numbers of cells were seeded in serum free medium and 0.1% BSA on top of transwell chambers coated with Matrigel. After 14 h, invaded cells were measured as described in Materials and Methods. Data are represented as the percentage of invaded cells, as mean±s.d. of triplicates (*p≤0.05).