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. 2013 Mar 22;8(3):e60086. doi: 10.1371/journal.pone.0060086

Figure 2. Dectin-1 specific ligands activate MSKs.

Figure 2

(A) BMDMs from wild type or dectin-1 knockout on a 129SvJ background were treated with 10 µg/ml of curdlan for the indicated times and the levels of phospho and total ERK1/2, phospho and total p38α and phospho CREB/ATF1 determined by immunoblotting. (B) As (A) except cells were stimulated with 200 µg/ml of depleted zymosan. (C) Where indicated, wild type BMDMs were treated with 2 µM PD184352 or 5 µM SB203580 for 1 h. Cells were then stimulated with 10 µg/ml of curdlan for 30 min and the levels of total and phospho (T581) MSK1, phospho ERK1/2, phospho and total p38α and phospho CREB/ATF1 determined by immunoblotting. (D) Wild type or MSK1/2 knockout BMDMs were stimulated with 10 µg/ml of curdlan for the indicated times and the levels of the indicated proteins determined by immunoblotting. The blots shown are representative of one (B) or two (A, C, D) separate experiments.