Table 1. The methylation indices (%) for the H19-DMR.

	Cases 1−43	Cases 44−138	Control subjects
Pyrosequencing analysis
CG5	4–24	35−50	33−48
CG6	5–26	36−53	34−51
CG7	4–24	35−49	30−47
CG9	5–23	34−48	30−46
COBRA
CG5 (Hpy188I)	3.3−35.1	37.8−60.8	36.2−58.5
CG16 (AflIII)	4.1−35.0	43.0−59.4	38.7−60.0

The position of examined CpG dinucleotides (CG5–7, CG9, and CG16) is shown in Figure 1A.

COBRA: combined bisulfite restriction analysis.