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. 2013 Mar 22;8(3):e59397. doi: 10.1371/journal.pone.0059397

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© 2013 Lu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Heat map of differentially regulated genes at day 8 of the eosinophil differentiation culture. Red: up-regulated in miR-21^−/− eosinophil progenitor culture compared to miR-21^+/+ eosinophil progenitor culture. (B) Heat map of differentially regulated genes at day 12 of the eosinophil differentiation culture. Red: up-regulated in miR-21^−/− eosinophil progenitor culture compared to miR-21^+/+ eosinophil progenitor culture; blue: down-regulated in miR-21^−/− eosinophil progenitor culture compared to miR-21^+/+ eosinophil progenitor culture. (C) Quantitative RT-PCR verification of a selected set of differentially expressed genes between miR-21^+/+ and miR-21^−/− eosinophil progenitor cultures. (D) Western blot showing levels of PSRC1 in miR-21^+/+ and miR-21^−/− eosinophil progenitor cultures at day 8 and day 12. GAPDH is shown as a loading control. (E) Functional enrichment analysis of differentially regulated genes in the eosinophil progenitor cultures at day 12. The networks are shown as Cytoscape graph networks generated from ToppCluster network analysis.