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Co-localization of PPP1CC2 and PPP1R2/PPP1R2P3 in morphologically normal and abnormal spermatozoa. Human spermatozoa were labeled with rabbit anti-PPP1CC2 and sheep anti-PPP1R2 antibodies, and specific secondary antibodies conjugated with Texas-Red and FITC fluorophores, respectively. (A), Negative control using only the fluorescence labeled secondary antibodies. (B), Spermatozoa with rabbit anti-PPP1CC2 and sheep anti-PPP1R2 antibodies. Phase contrast (PH). Scale bar = 20 μm.
