Table 1.

Peptides identified by Orbitrap Velos mass spectrometry for PPP1R2, PPP1R2P3 or both and for PPP1CC2, after immunoprecipitation of human sperm samples with sheep or rabbit anti-PPP1R2 antibodies. aa, amino acids; pI, isoelectric point

Protein name	Uniprot ID	MW (Da)	pI	Protein size (aa)	Coverage	Mascot score
PPP1R2	IPP2_HUMAN	23.015	4.64	205	22.0%	74.12
Peptide		Range (start-end)	Number of spectra	m/z meas.	z	Mascot score
K.WDEMNILATYHPADKDYGLMK.I		47–67	3	848.39	3	47.40
K.TSTTSSMVASAEQPRGNVDEELSK.K		19–42	2	847.40	3	26.72
Protein Name	Uniprot ID	MW (Da)	pI	Protein size (aa)	Coverage	Mascot score
PPP1R2P3	IPP2M_HUMAN	23.048	4.82	205	7.3%	48.95
Peptide		Range (start-end)	Number of spectra	m/z meas.	z	Mascot score
K.WDEINILATYHPADK.G		47–61	3	595.96	3	48.95
Protein Name		MW (Da)	pI	Protein size (aa)	Coverage	Mascot score
Common to both PPP1R2 and PPP1R2P3					49.3%	546.34
Peptide		Range (start-end)	Number of spectra	m/z meas.	z	Mascot score
R.KLAAAEGLEPK.Y		103–113	7	563.83	2	70.38
R.IQEQESSGEEDSDLSPEER.E		116–134	5	1082.46	2	117.82
K.LAAAEGLEPK.Y		104–113	5	499.78	2	63.21
K.LHYNEGLNIK.L		146–155	4	400.88	3	53.17
R.KLHYNEGLNIK.L		145–155	4	664.87	2	51.25
K.LHYNEGLNIKLAR.Q		146–158	2	514.29	3	23.57
R.IQEQESSGEEDSDLSPEEREK.K		116–136	2	807.69	3	59.32
K.DLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNK.L		164–201	1	1434.23	3	30.63
K.YRIQEQESSGEEDSDLSPEER.E		114–134	1	828.70	3	11.42
K.LAAAEGLEPKYR.I		104–115	1	439.91	3	15.97
K.TSTTSSMVASAEQPR.G		19–33	1	784.86	2	49.60
Protein Name	Uniprot ID	MW (Da)	pI	Protein size (aa)	Coverage	Mascot score
PPP1CC2	PP1G_HUMAN	38.518	5.78	337	15%	252.57
Peptide		Range (start-end)	Number of spectra	m/z meas.	z	Mascot score
R.VASGLNPSIQK.A		315–325	5	557.32	2	54.76
R.GVSFTFGAEVVAK.F		222–234	2	656.35	2	72.94
K.NVQLQENEIR.G		27–36	2	621.83	2	42.11
K.YPENFFLLR.G		114–122	1	599.82	2	39.6
K.LNIDSIIQR.L		7–15	1	536.31	2	43.16