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. 2013 Mar 22;8(3):e60103. doi: 10.1371/journal.pone.0060103

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© 2013 Mildner et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative CD31 stainings of wounds underneath the original wound edge are shown. Scale bars: 50 µm (B) The numbers of CD31 positive cells underneath the newly formed epidermis were evaluated. The graph represents the mean of 15 animals in each group (*: p<0.01). (C) The area of the granulation tissue taken up by CD31⁺ cells was evaluated. The graph represents the mean of 15 animals in each group. (D) Cell cycle analysis of SEC^PBMC treated microvascular EC shows a strong increase in proliferating cells. VEGF treatment served as positive control. One representative experiment of three each done in triplicates is shown (*: p<0.01). (E) A tube formation assay is shown. Compared to medium alone SEC^PBMC strongly induced tube formation in a matrigel assay. One representative experiment of three is shown.