Table 1. List of Antibodies.
Antigen | Company | No. | Dilution | |
Western blot | Immuno-staining | |||
CD31 | BD Biosciences1 | 553710 | n.d. | 1∶50 |
Ki67 | Novus Biologicals2 | NB500-170 | n.d. | 1∶50 |
Ki67 | Abcam3 | AB15580 | n.d. | 1∶1000 |
c-Jun | NEB4 | 9165 | 1∶100 | n.d. |
phospho-c-Jun | NEB4 | 9261 | 1∶100 | n.d. |
CREB | NEB4 | 9197 | 1∶100 | n.d. |
phospho-CREB | NEB4 | 9198 | 1∶100 | n.d. |
Akt | NEB4 | 2938 | 1∶100 | n.d. |
phospho-Akt | NEB4 | 9271 | 1∶100 | n.d. |
Erk1/2 | NEB4 | 4695 | 1∶100 | n.d. |
phospho-Erk1/2 | NEB4 | 4376 | 1∶100 | n.d. |
Hsp27 | NEB4 | 2402 | 1∶100 | n.d. |
phospho-Hsp27 | NEB4 | 2404 | 1∶100 | n.d. |
GAPDH | HyTest5 | 5G4 | 1∶2000 | n.d. |
mouse IgG HRP | Amersham6 | NA931V | 1∶10000 | n.d. |
rabbit IgG HRP | Thermo Fisher7 | 31460 | 1∶10000 | n.d. |
rabbit Fluor546 | Alexa8 | A-11035 | n.d. | 1∶500 |
rat igG HRP | Amersham6 | NA9350 | n.d. | 1∶500 |
Company addresses:
Bedford, MA, USA; 2Littleton, CO, USA; 3Cambridge, UK; 4NEB: New England Biolabs, Beverly, MA, USA; 5Turku Finland; 6Buckinghamshire, UK; 7Rockford, IL, USA; 8Eugene, OR, USA. n.d.: not done.