Figure 5.

Time course of the proteolytic activities of Ae. albopictus pupae and influence of pH on the proteolytic profile. A. Peptidase activities were detected after 12, 24, 36 and 48 h incubation of the gels at 37°C in 0.1 M Tris–HCl buffer (pH 7.5). B. The effect of pH on the proteolytic activities was evaluated by incubation at 37°C for 36 hours in 0.1 M sodium acetate buffer pH 3.5, 5,5 or 0.1 M Tris–HCl buffer pH 7.5, 10.0. The numbers on the right indicate the apparent molecular masses of the peptidases (kDa).