Figure 5. Binding of soluble ^BaLCHO gp120 to protein-A captured 2G12(A), VRC01 (B) and b12 (C) mAb preparations by SPR analysis.

^BaLCHO gp120 was injected at t = 0 for 180 s at a concentration of 100 nM, followed by a 450 second dissociation. Blank subtracted curves were normalized to the antibody capture levels for direct comparison. Insert: Normalized overlay plot for two CHO (red) selected b12 preparations to illustrate qualitative differences in the dissociation phase.