Table 1. Numerical results from the SPR analysis of HIV-BAL env binding.
Sample | Activity | k ass | k diss | K D | Rmax | Chi2 |
% | [105•M−1s−1] | [10−4. s−1] | [nM] | [RU] | ||
CHO2G12 1 | 108 | 1.2 | 1.03 | 0.86 | 74.7 | 1.42 |
CHO2G12 | = 100 | 1.18 | 1.31 | 1.11 | 67.9 | 0.394 |
p19/Nb2G12KDEL day 6 | 101 | 0.983 | 1.44 | 1.46 | 68.8 | 0.365 |
p19/Nb2G12KDEL day 12 | 102 | 0.931 | 1.82 | 1.95 | 69.2 | 0.354 |
p19/Nb2G12KDEL day 16 | 94 | 0.922 | 1.90 | 2.06 | 63.5 | 0.308 |
HEKVRC01 | = 100 | 0.719 | 0.581 | 0.807 | 78.1 | 0.025 |
SR1−NtVRC01KDEL day 4 | 83 | 0.740 | 0.551 | 0.745 | 65.0 | 0.009 |
p19/NbVRC01KDEL day 6 | 83 | 0.699 | 0.884 | 1.27 | 65.2 | 0.008 |
p19/NbVRC01KDEL day 12 | 77 | 0.811 | 1.00 | 1.23 | 60.1 | 0.020 |
p19/NbVRC01KDEL day 16 | 80 | 0.698 | 0.959 | 1.37 | 62.70 | 0.012 |
SR1−NtVRC01 day 4 | 75 | 0.618 | 0.715 | 1.16 | 58.60 | 0.022 |
p19/NbVRC01 day 6 | 78 | 0.668 | 0.970 | 1.45 | 60.70 | 0.011 |
p19/NbVRC01 day12 | 75 | 0.673 | 1.03 | 1.52 | 58.60 | 0.015 |
CHOb12 | = 100 | N/A 2 | ||||
SR1−Ntb12KDEL day 4 | 92 | |||||
p19/Nbb12KDEL day 11 | 92 | |||||
p19/Nbb12KDEL day 14 | 89 | |||||
p19/Nbb12 day 6 | 103 | |||||
p19/Nbb12 day 11 | 98 | |||||
p19/Nbb12 day 14 | 78 |
As reported [38], the comparison to these measurements shows an excellent reproducibility, with marginal differences being within experimental limits.
N/A. = not applicable.