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. 2013 Mar 7;6:54. doi: 10.1186/1756-3305-6-54

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Copyright ©2013 Ibraim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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LPGs purified from Leishmania infantum and Leishmania braziliensis induce translocation of NFkB through TLRs. CHO cells expressing TLR2 (TLR2+), TLR4 (TLR4+), or neither (TLR2-/TLR4-) were either untreated (gray line) or exposed (black line) to LPS, Staphylococcus aureus (SA), L. infantum LPG (LPGi) or L. braziliensis LPG (LPGb), as indicated. CD25 expression was measured by flow cytometry 18 h after stimulation. Percentage = percentage of CD25 expression on stimulated cells minus percentage of CD25 expression on non-stimulated cells.