Table 3.
DNA migration in the comet assay observed in Wistar rat colon treated with different doses of the C. langsdorffii extract and/or DMH and their respective controls
|
Treatments (mg/kg) |
Comet class* |
*Score |
Reduction |
|||
|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | (%) | ||
| Control |
66.2 ± 4.3 |
23.2 ± 5.7 |
7.3 ± 2.3 |
3.3 ± 1.8 |
47.8 ± 6.4 |
- |
| DMSO |
65.3 ± 3.9 |
21.2 ± 4.4 |
8.3 ± 2.4 |
3.7 ± 2.2 |
48.8 ± 4.8 |
- |
| 80 |
79.7 ± 5.1 |
14.7 ± 6.3 |
5.0 ± 2.6 |
0.7 ± 0.8 |
26.7 ± 6.0 |
- |
| DMH |
13.2 ± 2.1 |
26.2 ± 5.3 |
33.5 ± 5.2 |
27.3 ± 3.7 |
175.2 ± 9.6a |
- |
| DMSO + DMH |
11.5 ± 1.6 |
23.8 ± 9.5 |
32.8 ± 8.3 |
28.7 ± 4.3 |
175.5 ± 11.7a |
- |
| 20 + DMH |
10.8 ± 4.5 |
40.2 ± 3.6 |
25.0 ± 6.4 |
22.3 ± 4.3 |
157.2 ± 12.2a,b |
14.3 |
| 40 + DMH |
19.3 ± 6.3 |
34.0 ± 4.3 |
29.7 ± 3.6 |
16.8 ± 5.9 |
143.8 ± 15.1a,b |
24.7 |
| 80 + DMH | 23.3 ± 6.9 | 40.8 ± 7.1 | 20.8 ± 3.5 | 15.0 ± 5.9 | 125.8 ± 12.7a,b,c | 38.8 |
A total of 600 cells were examined per treatment; DMH, 1,2-dimethylhydrazine (40 mg/kg). The numbers 20, 40, and 80 correspond to the extract dose in mg/kg.
*Values are the mean ± standard deviation.
aSignificantly different from the control (P < 0.05).
bSignificantly different from the DMH group (P <0.05).
cSignificantly different from the 20 + DMH group (P < 0.05).