DBA/2 mice were treated with G3139-LNP, free G3139 or empty LNPs, and then injected i.p. with BrdU. Three mice were in each group. Twenty four hours after treatment, spleen cells were harvested, and the activation status of DX5+ NK cells (A), CD11c+ DCs (B), CD4+ T cells (C), and CD8+ T cells (D) were evaluated by BrdU incorporation rate. Results represent the average ± SD of three independent experiments. (* indicates p < 0.05, by Student’s t test)