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. 2013 Mar 22;8(3):e59262. doi: 10.1371/journal.pone.0059262

Figure 5. The GH31 LGT is ancient in origin.

Figure 5

A.) Putative homologs of DpGH31 identified using degenerate PCR on genomic DNA from a panel of diverse lepidoptera species. Phylogenetic relationships of the families are taken from Cho et al 2011 [36]. Bombxy mori genomic DNA was used as the positive control (+). Faint bands could be observed for P. populi and A. velutinana on the original gel. B.) A Bayesian phylogenetic tree derived from GH31-LGT conceptually translated DNA sequences. DNA sequences used were obtained either by degenerate PCR or searches of available lepidopteran genome sequences. Well supported clades are resolved for members of the Noctuoidea and Geometroidea. The phylogenetic relationships of species from the Bombycoid complex and the butterflies could not be resolved in this analysis (posterior probability values less than 90%).