Table 1.

Rotenone-induced ultrastructural changes in SNc (semi-quantification)

	Normal	Mitochondrial swelling	Mitochondrial crest fracture	Mitochondrial vacuolar degeneration	Dilated and broken rough endoplasmic reticula	Lipofuscin deposition	Perinular space augmentation	increase of autophagic vacuoles/lysosomes	Degeneration of medullary sheathes
Contralateral SNc (Figure 6A)	Mitochondrial complex ribosomes, medullary sheathes
One day after surgery (Figure 6B)		+	+	+	+			+
Two day after surgery (6C)		++	++	++	++			++
One week after surgery (Figure6E)		+++	+++	+++		++
Two weeks after surgery (6F)		+++	+++	+++		++
Four weeks after surgery (Figure 6G,H)		+++	+++	+++	++		++	+	++

Rotenone-administrated animals were sacrificed 1 day, 2 days, 1 week, 2 weeks or 4 weeks after the stereological surgery. A 1-mm3 tissue block from the ipsilateral and contralateral SNc regions (−4.5 to −6.2 mm caudal to the bregma) was micro-punched for TEM assessment. “+”, frequency of rotenone-induced ultrastructural changes in SNc, including mitochondrial swelling, mitochondrial crest fracture, mitochondrial vacuolar degeneration, dilated and broken rough endoplasmic reticula, lipofuscin deposition, perinuclear space augmentation and increase in autophagic vacuoles.