Skip to main content
. 2013 Mar 22;8(3):e59389. doi: 10.1371/journal.pone.0059389

Figure 4. The −1.9 kb element is a NF-κB-dependent LPS inducible promoter and enhancer in transient transfection.

Figure 4

HD11 macrophages were transfected for 18 hrs with Jetpei 2 µl and 1 µg DNA prior to 7 hrs 1 µg/ml LPS stimulation, black bars, or remained untreated for 7 hrs, grey bars. The constructs are illustrated adjacent to the y-axis. (a) Clys promoter is a black arrow, the −1.9 kb CRE is a dark grey arrow, the firefly luciferase coding region is grey. (b) The −1.9 kb element is cloned in antisense orientation, Position of the NF-κB, C/EBP, X unknown protein and AP1 are indicated next to the Y axis. (a and b) The data are plotted as the mean value of two independent experiments, individual experiments had triplicate samples for each condition. Inter sample variation has been corrected by Renilla normalisation. Positive error bars indicate standard deviations.