Table 2.
Fatty acid concentration (μM) |
Glucose uptake |
|
---|---|---|
PA | PAM | |
Control (0) |
1.00a ± 0.045 |
1.00a ± 0.067 |
1 |
1.23b ± 0.105 |
1.09a ± .051 |
5 |
1.29b ± 0.071 |
|
10 |
1.32b ± 0.075 |
1.14a ± 0.061 |
20 |
1.34b ± 0.166 |
|
40 |
1.37b ± 0.076 |
|
50 |
|
0.92a ± 0.032 |
60 |
1.37b ± 0.079 |
|
100 |
|
0.75b ± 0.032 |
200 |
|
0.48c ± 0.04 |
400 | 0.32d ± 0.028 |
Glucose uptake was carried out on differentiated myotubes that had been incubated with 1–60 μM PA or 1and 10 μM PAM for 4 h. PAM from 50–400 μM was administered for 24 h. Control samples lacked fatty acids. Data are expressed as LSmean values of triplicate wells, carried out in three separate experiments with cells isolated from a different pig each time. LSmeans with different letters (a-d) within each row denote significantly different responses related to fatty acid treatments; (P < 0.05).