Table 1. Strains, plasmids and primers used in this study.
E. coli strain, plasmids, or primers | Relevant genotype and/or description | Source or reference |
Strains | ||
BW25113 | F- λ- Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) rph-1 hsdR514 Δ(rhaD-rhaB)568 | [29] |
JW0940 | BW25113::ompA::kanR | [29] |
FS20-pSU | MC4100::rpoS:: KanR; containing pSU, CmR | [13] |
FS20-pSURpoS | MC4100::rpoS:: KanR; containing pSURpoS, CmR | [13] |
Plasmid | ||
pDsRED | pUC replicon; red-fluorescence-protein (RFP) expressing plasmid; Ampr | [12] |
Primers | ||
ompA(CHK) Forward | cgaagatatcggtagagtt | This study |
ompA(CHK) Reverse | cgctttctgaaacgattgt | This study |
ompA (qPCR) Forward | cttcgctggcggtgttgag | This study |
ompA (qPCR) Reverse | acgagtgccgatggtgtgt | This study |
16S rDNA (qPCR) Forward | tgcatctgatactggcaagc | [13] |
16S rDNA (qPCR) Reverse | acctgagcgtcagtcttcgt | [13] |