Table 2.
Conservancy of neuraminidase (NA) epitopes of sH1N1 and pH1N1 influenza viruses
| NA sequence epitopea | Number (%) of study years conservedb | Epitope id in IEDB | Possible B-cell epitopec | Possible MHC I allelesd | Possible MHC II allelese |
|---|---|---|---|---|---|
| KDNSIRIGSKGDVFVIR |
99.8 |
129048 |
SIRIGSKGDV(105–114) |
A*0201,A*03, |
DRB1*0101,DRB1*0301,DRB1*0401 |
| (102–118) |
|
|
DNSIRIGSKGDVFV(103–116) |
A*1101 |
DRB1*0701,DRB1*1101,DRB1*1501 |
| IGSKGDVFVIREPFIS |
99.8 |
128924 |
GDVFVIREPF(112–121) |
A*0201,A*03, A*1101 |
DRB1*1101,DRB1*1501, |
| RTFFLTQGALLNDKHSN |
99.5 |
127810 |
FLTQGALLND(133–142) |
A*01,A*03, |
DRB1*0101,DRB1*0301,DRB1*0401 |
| (130–146) |
|
|
FFLTQGALLNDKHS(132–145) |
A*1101 |
DRB1*0701,DRB1*1101,DRB1*1501 |
| ISGPDNGAVAVLKYNGI |
99.5 |
128994 |
ISGPDNGAVAVLKY(195–208) |
A*01,A*0201 |
DRB1*0301,DRB1*0401,DRB1*1501 |
| (195–211) |
|
|
GPDNGAVAVL(197–206) |
|
|
| VCRDNWHGSNRPWVSFN |
99.9 |
130190 |
CRDNWHGSNRPW(292–303) |
A*01 |
DRB1*0101,DRB1*0401,DRB1*0701, |
| (291–307) |
|
|
RDNWHGSNRPWVSF(293–306) |
|
DRB1*1101 |
| WSGYSGSFVQHPELTGL |
99.9 |
130342 |
* |
A*0201,A*03, |
DRB1*0101,DRB1*0401,DRB1*0701, |
| (399–415) |
|
|
|
A*1101 |
DRB1*1101,DRB1*1501, |
| SFVQHPELTGLDCIRP |
99.2 |
129889 |
* |
A*01, A*0201, |
DRB1*0101,DRB1*0401,DRB1*1501 |
| (405–420) |
|
|
|
A*03, A*1101 |
|
| PELTGLDCIRPCFWVEL |
99.3 |
129560 |
* |
A*01,A*0201, |
DRB1*0101,DRB1*0301,DRB1*0401 |
| (410–426) | A*03,A*1101 | DRB1*0701,DRB1*1101,DRB1*1501 |
a. Epitopes in IEDB were experimentally identified epitopes in sH1N1 NA genes. b. The conservancy ratio of 1928 genes of sH1N1 NA sequences, whose strains isolated from 1968 to 2008. c. The B-cell epitopes of pH1N1 NA genes are predicted by ABCpred, respectively. d. MHC I alleles of 10 mer and more of pH1N1 NA genes are Predicted by BIMAS (explicit number = 100) and SYFPEITHI (score ≥10.0), respectively. The same epitope sequences predicted by both BIMAS and SYFPEITHI were determined. e. MHC II alleles of 10 mer and more of pH1N1 NA genes are predicted by SYFPEITHI (score ≥10.0), respectively.