Table 3.
Summary of chemical shifts and J 1,2 coupling constants of anomeric protons in complex GSLs purified from A. franciscana
| CHpS1 | GalNAc1-3,4 | (GlcNAc1- | 2Fuc1-3,4) | GlcNAc1- | 3GlcNAc1- | 3Man1- | 4Glc1 | |||
| II | V | V | IV | IV | III | I | ||||
| Chemical shifts (ppm) | 4.29 | 4.98 | 4.98 | 4.60 | 4.60 | 4.52 | 4.16 | |||
| 4.99 | 4.99 | 4.63 | 4.63 | |||||||
| Coupling constants (Hz) | 8.2 | 2.7 | 2.7 | 8.2 | 8.2 | ∼1 | 7.8 | |||
| 3.2 | 3.2 | 9.1 | 9.1 | |||||||
| CHpS2 | GlcNAc1-3 | GalNAc1-4 | (GlcNAc1- | 2Fuc1-3) | GlcNAc1- | 3Man1- | 4Glc1 | |||
| IV | II | V | V | IV | III | I | ||||
| Chemical shifts (ppm) | 4.58 | 4.35 | 5.01 | 5.01 | 4.58 | 4.51 | 4.15 | |||
| 4.65 | 5.02 | 5.02 | 4.65 | |||||||
| Coupling constants (Hz) | 7.3 | 7.8 | 3.4 | 3.4 | 7.3 | ∼1 | 7.8 | |||
| 7.8 | 3.6 | 3.6 | 7.8 | |||||||
| CNS | GalNAc1-4 | (Fuc1-3) | GlcNAc1-3 | GalNAc1-4 | (GlcNAc1- | 2Fuc1-3) | GlcNAc1- | 3Man1- | 4Glc1 | |
| II | VI | IV | II | V | V | IV | III | I | ||
| Chemical shifts (ppm) | 4.32 | 4.84 | 4.63 | 4.32 | 5.01 | 5.01 | 4.63 | 4.52 | 4.15 | |
| 4.34 | 4.64 | 4.34 | 4.64 | 4.16 | ||||||
| Coupling constants (Hz) | 6.9 | 3.7 | 7.3 | 6.9 | 2.7 | 2.7 | 7.3 | ∼1 | 6.4 | |
| 7.8 | 7.3 | 7.8 | 7.3 | 8.7 | ||||||
| CDeS | GalNAc1-4 | (GlcNAc1- | 2Fuc1-3) | GlcNAc1-3 | GalNAc1-4 | (GlcNAc1- | 2Fuc1-3) | GlcNAc1- | 3Man1- | 4Glc1 |
| II | V | V | IV | II | V | V | IV | III | I | |
| Chemical shifts (ppm) | 4.34 | 5.02 | 5.02 | 4.64 | 4.34 | 5.02 | 5.02 | 4.64 | 4.51 | 4.15 |
| 4.37 | 4.68 | 4.37 | 4.68 | |||||||
| Coupling constants (Hz) | 8.0 | 2.9 | 2.9 | 7.2 | 8.0 | 2.9 | 2.9 | 7.2 | ∼1 | 7.6 |
| 8.1 | 7.4 | 8.1 | 7.4 | |||||||