Fig. 3.

Lectin- and Western-blot analysis of L1CAM glycosylation. Immunoprecipitated L1CAM from primary WM793 and metastatic WM1205Lu cell lines was probed with a set of lectins a. Immunodetection of L1CAM from primary WM793 and metastatic WM1205Lu cell lines in lectin precipitates, using anti-L1CAM mAb (UJ127.11; 1: 12500) b. Lectin abbreviations and binding specificities are as follows: GNA, Galanthus nivalis agglutinin (recognises high mannose and hybrid-type oligosaccharides); DSA, Datura stramonium agglutinin (recognises two or three N-acetyllactosaminyl repeats); LEA, Lycopersicon esculentum agglutinin (recognises three or more N-acetyllactosaminyl repeats); PHA-L, Phaseolus vulgaris leucoagglutinin (recognises GlcNAcβ1-6-branched triantennary and tetraantennary complex-type glycans); PHA-E, Phaseolus vulgaris erythroagglutinin (recognises bisecting GlcNAc residue); MAA, Maakia amurensis agglutinin (recognises sialic acid α2-3-bound to terminal galactose residue); SNA, Sambucus nigra agglutinin (recognises sialic acid α2-6-bound to terminal galactose); AAA, Aleuria aurantia agglutinin (recognises fucose residues); UEA-I, Ulex europaeus I agglutinin (recognises fucose α1-2-bound to terminal galactose residue). CE, immunodetection of L1CAM in whole cell extract