Table 5.
Physiological characteristics, metabolic parameters, and immune cell phenotyping in ApoE-KO mice transplanted with ApoE-KO or DKO BM cells
| Parameter | ApoE-KO BM → ApoE-KO | DKO BM → ApoE-KO |
|---|---|---|
| Body weight (g) | 32.4 ± 2.2 | 36.4 ± 2.6 |
| Subcutaneous adipose tissue weight, absolute weight (mg) | 809.7 ± 249.5 | 1106.4 ± 218.2 |
| Epididymal adipose tissue weight, absolute weight (mg) | 1091.3 ± 317.2 | 1481.3 ± 285.7 |
| Mesenteric adipose tissue weight, absolute weight (mg) | 364.1.3 ± 70.9 | 606.1 ± 135.0 |
| Spleen weight ratio (mg/g BW) | 4.56 ± 0.41 | 4.51 ± 0.73 |
| Thymus weight ratio (mg/g BW) | 1.12 ± 0.17 | 0.92 ± 0.16 |
| Heart weight ratio (mg/g BW) | 4.88 ± 0.44 | 4.68 ± 0.38 |
| Left kidney weight ratio (mg/g BW) | 6.19 ± 0.41 | 5.65 ± 0.23 |
| Monocytes in blood (105 cells/ml) | 10.8 ± 1.7 | 10.9 ± 1.7 |
| Neutrophils in blood (105 cells/ml) | 22.3 ± 6.5 | 16.2 ± 2.9 |
| T lymphocytes in blood (105 cells/ml) | 16.6 ± 1.8 | 14.7 ± 1.0 |
| B lymphocytes in blood (105 cells/ml) | 69.4 ± 4.2 | 58.7 ± 7.8 |
| CD45+11β-HSD1+ in blood (105 cells/ml) | 60.5 ± 10.6 | 3.2 ± 0.7*** |
| BM monocytes (105 cells/femur) | 7.1 ± 2.3 | 8.3 ± 1.9 |
| BM neutrophils (105 cells/femur) | 35.8 ± 11.8 | 53.2.±14.4 |
| BM CD45+11β-HSD1+ (105 cells/femur) | 50.0 ± 16.2 | 1.8 ± 0.4* |
| Splenic monocytes (105 cells/femur) | 5.2 ± 1.7 | 9.2 ± 3.2 |
| Splenic neutrophils (105 cells/femur) | 6.2 ± 1.4 | 9.6 ± 2.6 |
| Splenic T lymphocytes (105 cells/ml) | 10.0 ± 2.8 | 12.1 ± 4.9 |
| Splenic B lymphocytes (105 cells/ml) | 22.5 ± 7.3 | 24.2 ± 8.8 |
Data are expressed as means ± se; n = 5/group. BW, body weight.
***
P < 0.001 vs. ApoE-KO BM → ApoE-KO.