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. 2013 Apr;27(4):1532–1548. doi: 10.1096/fj.12-219600

Figure 4.

Figure 4.

Involvement of VEGF-R1 in Tat-mediated increase of primary rat microglial adhesion/migration. A) Schematic of Tat domains. B, C) Primary rat microglia were treated with different Tat domains: Tat1-15, Tat46-60, mutant Tat1-72Δ31-61, or heated Tat for 6 h followed by microglial adhesion (B) and migration assay (C). Data are presented as means ± se of 4 independent experiments. **P < 0.01, ***P < 0.001 vs. control group. D) Detection of VEGF-R1 and R2 mRNA by RT-PCR analysis in primary rat microglia. E) Tat-mediated phosphorylation of VEGF-R1 in primary rat microglia. F, G) Effect of neutralizing VEGF-R1 antibody (1 and 10 μg/ml) on Tat-mediated MLC phosphorylation (F) and β1 integrin activation (G). H, I) Effect of neutralizing VEGF-R1 antibody on Tat-mediated primary rat microglial adhesion (H) and migration (I). Data are presented as means ± se of 4 independent experiments. ***P < 0.001 vs. control group; ##P < 0.01, ###P < 0.001 vs. Tat-treated group.