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. 2013 Feb 22;69(Pt 3):270–274. doi: 10.1107/S1744309113001917

Figure 2.

Figure 2

Nanobody production. Size-exclusion chromatography elution profile of the recombinant Nb2907 (red) and Nb2919 (black) on a Superdex HR 16/60 column equilibrated with a buffer consisting of 50 mM Tris–HCl pH 7.5, 150 mM NaCl. The blue squares represent the standard used to determine the relationship between elution volume and molecular weight and consists of vitamin B12 (1.35 kDa), ubiquitin (8.6 kDa), myoglobin (17 kDa), DBL6∊ (38 kDa) and ovalbumin (44 kDa). Inset: SDS–PAGE of PageRuler Prestained Protein Ladder (lane 1, Thermo Scientific), purified Nb2907 (lane 2) and Nb2919 (lane 3) on 15% SDS–PAGE.