Table 2. Comparison of elution volumes of the mutants and standard proteins.
| Protein | Theoretical molecular mass (Da) | Elution volume (ml) |
|---|---|---|
| Standard | ||
| Ovalbumin | 44 000 | 63.9 |
| Carbonic anhydrase | 29 000 | 70.4 |
| Chymotrypsinogen | 25 000 | 71.5 |
| Sample | ||
| R167AD4 | 25 246 | 67.6 |
| 3GD4 | 25 190 | 63.6 |
| Δ171-172D4 | 26 998 | 63.4 |