Table 2. Purification of recombinant methicillin-resistant S. aureus N-acetylneuraminate lyase from a 1.5 l culture.

Purification step	Total protein (mg)	Specific activity (µmol min−1 mg−1)	Total activity (µmol min−1)	Yield (%)
Crude	408	6.5	2646	100
Anion exchange	177	8.2	1443	55
Hydrophobic interaction	34.4	19	669	25
Size exclusion	32.8	11	374	14