Table 1.
Statistics for 454 pyrosequencing libraries
| Skeletal muscle | Intestine | Blood | HK-38 | HK-105 | |
|---|---|---|---|---|---|
|
Pyrosequencing reads |
|
|
|
|
|
| High-quality reads |
447,166 |
614,899 |
576,796 |
577,112 |
729,941 |
| Average read length (bp) |
294 |
304 |
290 |
349 |
320 |
| Total Megabases |
131.5 |
186.9 |
167.3 |
201.4 |
233.6 |
|
Assembly statistics |
|
|
|
|
|
| Number of contigs |
7,808 |
9,475 |
12,003 |
14,008 |
12,474 |
| Reads assembled |
314,628 |
482,080 |
389,934 |
392,195 |
508,826 |
| Average contig length (bp) |
968 |
1,090 |
831 |
1,059 |
1,148 |
| Number of singletons |
48,750 |
80,527 |
114,984 |
105,431 |
91,885 |
| Average singleton length (nt) |
316 |
298 |
284 |
318 |
334 |
| Total consensus Megabases |
23.0 |
34.3 |
42.7 |
48.4 |
45.0 |
| Average sequences coverage | 6.4 | 6.3 | 4.0 | 4.2 | 5.8 |
Sequence characteristics of the five 454 normalized libraries derived from skeletal muscle, intestine, blood and head kidney (HK-38, early parasite infection; HK 105, chronic parasite infection).