Table 1.
List of genes selected for differential expression analysis and the oligonucleotides used in this study
| Putative function | Accessionnumber | E-Value | Protein ID* | qPCR forward (F) and reverse (R) primers (5' to 3') |
|---|---|---|---|---|
| acid sphingomielinase (asm) |
JK840922 |
1.39E-136 |
548323 |
Forward: GCGAAGCATCTCGGCTATTGTAGT |
| Reverse: TCAAGTTGTGAACCGCTACTCGTC | ||||
| β-1,3-endoglucanase (bgn) |
JK840920 |
8.92E-21 |
241696 |
Forward: TCAACATCGCCAACGTCAACGAC |
| Reverse: TGCCAATACGGGAACCAGTGATC | ||||
| chitinase 33 (chit) |
JK840912 |
3e-51 |
387920 |
Forward: TGGAGCTCAACAGGCGCTGC |
| Reverse: ACGACGGCACTGCCAAAGGG | ||||
| endochitinase 42 (endo) |
JK840909 |
3.0E-46 |
364419 |
Forward: AAGGGTTACTACAGCTACAACGCC |
| Reverse: ACTTGAGGTAGGCAACCTTGGTGT | ||||
| exo-rhamnogalacturonase (exo) |
JK840947 |
2.12E-65 |
463001 |
Forward: TTACCTGAAGACATGGGCGGGAAT |
| Reverse: GCCTTCCGCCAATCAGCTTAACAT | ||||
| glycosyl hydrolase (glyc) |
JK840945 |
1.51E-09 |
199282 |
Forward: GAAATGTTGTCGTCACCAGACGGT |
| Reverse: GGCCGCGATTGCTGTTTCATAGT | ||||
| amine oxidase (aoc) |
JK840953 |
9.50E-15 |
538657 |
Forward: ATACACCCGAAGGAACCTTGTTGG |
| Reverse: TAGCGTGCCTCAATCTCCTTAGCA | ||||
| phospholipase d (pld) |
JK840907 |
3.58E-56 |
537712 |
Forward: TGGGAAGACGTTGCACACACAAAC |
| Reverse: AAATTGTCGTAGTCGTCCCAGGTG | ||||
| checkpoint-like protein (chk1) |
JK840936 |
2.48E-14 |
NA |
Forward: TGCTGCCTTCCTTGGATGTAGTAG |
| Reverse: AAACATGGTGGCAACGGGTAACG | ||||
| serine threonine-protein kinase (sck1) |
JK840919 |
1.15E-80 |
480202 |
Forward: ATGCTGAAGAGCTTAAACGCCACC |
| Reverse: ACTTTGGCTTGAAGGGTGGAGAG | ||||
| senescence-associated protein (sag) |
JK840934 |
5.02E-30 |
547464 |
Forward: AGCTCACGTTCCCTATTAGTGGGT |
| Reverse: ATCCTTCGATGTCGGCTCTTCCTA | ||||
| mbl2-like secreted (mbl2) |
JK840948 |
2.05E-41 |
257052 |
Forward: TTGCTACGAGGGAGTTTGTTCCTG |
| Reverse: TGGAGTTGCACTGGTCTGAAGT | ||||
| serine protease (ser) |
JK840930 |
1.54E-12 |
366985 |
Forward: TGGAAGGGAGTGACCAAGCCTG |
| Reverse: GGAAAGGTCAGGAGTGCTATCGGG | ||||
| aquaporin (aqp) |
JK840978 |
1.47E-60 |
476226 |
Forward: GTTGATGGCATAACCAGTCTCCCA |
| Reverse: CAACAACATTGGAGCCGGAAACCT | ||||
| duf895 domain membrane protein (duf) |
JK8409133 |
1.67E-92 |
396055 |
Forward: TCCAATCCTTGCCGACGTAGTTGA |
| Reverse: TGCCAAGATCACATGGGTCGTTCT | ||||
| peptide transporter (ptr2) |
JK840963 |
8.11e-74 |
533699 |
Forward: AGTCATCTGGTTGTAGGCCAGGAA |
| Reverse: AAATTGTCGTAGTCGTCCCAGGTG | ||||
| QID74 protein (qid) |
JK840906 |
6.31E-113 |
456637 |
Forward: CAGAAGAAGTGCGTGTGCAACAAG |
| Reverse: AGCTAGCATCTTTGCCGCAGTTTG | ||||
| eight cysteine-containing domain (cfem) |
JK840940 |
1.09E-17 |
245062 |
Forward: GCGTCCGCAAAGAAACAACCTTCT |
| Reverse: AGAGAGCGGTGTTTGTAGCGATGA | ||||
| Enolase (eno) |
JK841017 |
3.54E-12 |
315824 |
Forward: ACTTTGACCGAGTCTATCCAGGCT |
| Reverse: ATACCGACGGAGATGTCAGCAATG | ||||
| glyceraldehyde 3- phosphate dehydrogenase (gapd) |
JK840981 |
1.32E-32 |
281265 |
Forward: CAGGTCGCCAAGAAGGTCATCATT |
| Reverse: AAGCGTTGGAGATGACATTGGCAC | ||||
| pyruvate decarboxylase (pdc) |
JK840980 |
2.60E-31 |
277935 |
Forward: GCAGGTGTTGGTCAATTCCTTCAG |
| Reverse: AACGCCAGATGGGAACTTGGTATC | ||||
| heat shock protein (hsp98) |
JK841007 |
9.91E-13 |
225475 |
Forward: TTGAGCGTCGTTTCCAACAGGTTC |
| Reverse: TGTCGAGAATGCTGACCTTGTGGT | ||||
| zinc finger domain protein (c2h2) |
JK841004 |
1.67E-15 |
543717 |
Forward: CAGACCTTGCACTTGTGCTTCTT |
| Reverse: AATGTTGTCGACCTCACTGCCT | ||||
| hexose transporter-like protein (ht) |
JK840987 |
1.20E-35 |
360713 |
Forward: GGAGTCCCATTTGCTCGAAGTGAT |
| Reverse: CGTGCTCATCGTCTTCTT CTTCGT | ||||
| zinc-regulated transporter (zt) |
JK840990 |
3.84E-15 |
295941 |
Forward: GGCCAAGAAATCCAGCAGGAAGAT |
| Reverse: TTGACTAGTGGCTGGGCTGAATAC | ||||
| norsolorinic acid reductase (norA) |
JK841015 |
6.41E-37 |
373869 |
Forward: ACCGTCTCGTCAACATGAGCTACT |
| Reverse: AAGTTCATGGAACCCAAGCACAGC | ||||
| phosphatidylserine decarboxylase family protein (psd) |
JK840982 |
4.91E-23 |
363281 |
Forward: TCTTTGAAGGCGTAGGTGATCCGA |
| Reverse: GCAGATCCGTAGAGGCAAGTATGT | ||||
| coproporphyrinogen oxidase (cpox) |
JK841003 |
4.88E-24 |
364552 |
Forward: TGATCCAGGCGTGTTCAGTCCAAA |
| Reverse: GCAGATCCGTAGAGGCAAGTATGT | ||||
| α-tubulin |
HS574101 |
|
|
Forward: TATCTGCTACCAGGCTCCCGAGAA |
| Reverse: TGGTGTTGGACAGCATGCAGACAG |
* BLASTX at http://genome.jgi.doe.gov/ using Trichoderma harzianum CBS 226.95 as a reference model.