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Western blot profile of CSA probed with representative individual human serum. Lane MW: Molecular weight standard (PageRulerTM Prestained Protein Ladder); Lane 1: Pooled human ALA serum; Lane 2: Pooled human IHA seronegative serum; Lanes 3 to 9: Individual human ALA serum; Lanes 10–12: Individual human IHA seronegative serum; Lane 13: TBS control. (*) indicates the estimated molecular weight of proteins (~170 kDa, ~100 kDa and ~70 kDa).
