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. Author manuscript; available in PMC: 2014 Mar 22.
Published in final edited form as: J Nat Prod. 2012 Dec 19;76(3):445–449. doi: 10.1021/np300766p

Figure 2.

Figure 2

Western blot analysis of glioblastoma cell line U251 following treatment with withanolides 1–5. Total levels of HSP32 and HSP70, biomarkers of cellular stress and withanolide activity, were screened at 0, 0.1, 0.5, 1, and 5 μM concentrations of each compound for 24h. HSP70 was markedly upregulated at 5 μM with withanolides 2, 3, and 5, while HSP32 levels were elevated in a concentration-dependent manner with all compounds 1–5 tested. The β-actin protein was used as a loading control marker.