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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Nature. 2013 Feb 10;495(7441):370–374. doi: 10.1038/nature11925

Fig. 2. Synergy between Nanog and Tet1 during reprogramming.

Fig. 2

a, Nanog and Tet1 are specifically expressed in pluripotent cells. b, Knockdown of Tet1 compromises reprogramming activity of a constitutive Nanog transgene in reprogramming intermediates. c, Both wild-type and mutant Tet1 enhance Nanog-dependent reprogramming. Quantification of the number of iPS colonies at day 10 of 2i/LIF treatment in Supplementary Fig. 10b is shown. d-e, Contribution of iPS cells generated with Nanog and Tet1WT (top) or Tet1Mut (bottom) transgenes to the germline at E12.5 (d) and live-born chimeras (e). siTet1, siRNA against Tet1; siNT, non-targeting siRNA control; +rOKM, adult NS cells transduced with retroviral Oct4, Klf4, and c-Myc transgenes. Error bars indicate standard deviation (n=3).