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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Nature. 2013 Feb 10;495(7441):370–374. doi: 10.1038/nature11925

Fig. 3. Synergy between Nanog and Tet1/2 during reprogramming is dependent upon catalytic activity of Tet1/2.

Fig. 3

a, Measurement of global levels of 5hmC (left) and Tet2 expression (right) in reprogramming intermediates transfected with PB transgenes. b, Schematic depiction of wild-type (WT) Tet1, Tet2, and the truncated Tet1 mutant (Tet1C). Note the absence of a CXXC DNA binding domain in Tet2 and Tet1C proteins. c, Quantification of GFP+ iPS colonies. d, Physical association of Nanog with Tet1C. CoIP was performed in HEK293T cells. e, Tet2 knockdown (siTet2) reduces reprogramming efficiency in intermediate cells transgenic for Nanog+Tet1Mut compared to Nanog+Tet1WT. Non-targeting siRNA (siNT) serves as a control. f, Quantification of the number of iPS colonies in (e). Error bars indicate standard deviation (n=3). CD, catalytic domain.