Figure 3.
Mean protein levels (with representative immunoblots) of A, COX-2; D, Cytochrome p450 epoxygenase; G, BDNF and J, CREB. Bar graphs are ratios of optical densities of individual protein bands to β-actin, expressed as percent of control. Mean mRNA levels of B, COX-2; E, Cytochrome p450 epoxygenase; H, BDNF and K, CREB in postmortem frontal cortex from the middle aged (n = 9) and aged group subjects (n = 10), measured using quantitative RT-PCR. -mRNA levels of COX-2, Cytochrome p450 epoxygenase, BDNF and CREB in aged group normalized to the endogenous control (β-globulin) and relative to the control (calibrator), using the ΔΔCT method. Mean levels of CpG promoter methylation at C, COX-2; F, Cytochrome p450 epoxygenase; I, BDNF and L, CREB. Values are Mean ± SEM and t-tests are used to compare old vs. middle age groups. * p < 0.05, ** p < 0.01, *** p < 0.001 as compared to aged group.