. 2013 Jan 26;6(2):63–76. doi: 10.1007/s12154-012-0090-1

Table 1.

Identities and relative amounts (optical density) of proteins present

ID	Band	Observed mass^a (kDa)	Matching peptides found in MS/MS	Database entry number and species	Closest homologue in Viridiplantae	Optical density	±^b
Caleosin A	F	28.1	VAFDPFGWTAAK	EL461280	Caleosin; Sesamum indicum, gi\|6478218	3.1 %	0.31 %
			MEWAILYALAK	H. tuberosus
			CFDGSLFDYCAK
			TDLETHLPKPYLAR
			TVPDKLTLGEIWNMTEGNR
Caleosin B	G	26.3	MEWAILYALAK	EL460587	Caleosin; S. indicum, gi\|6478218	5.7 %	0.54 %
			CFDGSLFDYCAK	H. tuberosus
			YMPVHFENMFSK
			TDLETHLPKPYLAR
			GLVAPDTEHPNGTLGHR
			TVPDKLTLGEIWNMTEGNR
			VMAMADGNALTPEADCQPLTHER
Hydrosteroid 11-β-dehydrogenase A (steroleosin A)	A, B, D, E	45 (×2), 36.2, 33.4	AALAQFFETMR	GE513976	Steroleosin; S. indicum, gi\|16033752	9.2 %	1.95 %
			MVDQTVHHFNR	H. annuus
			IIALSSAASWIPLPR
			ITLVTPGFIESELTQGK
			VVLITGASSGIGEHIAYEYASR
Hydrosteroid 11-β-dehydrogenase B (steroleosin B)	C	38.2	AALVNFYETLR	EL457176	Steroleosin-B; S. indicum, gi\|21311775	3.5 %	0.01 %
			FINETINYFGR	H. tuberosus
			LLGAPDVLIMAADVVK
			IVVNAAVENWLPLPR
Oleosin A	J	18.2	GGHYGTAGVHTGGPGGGVGTYV	CD856507	Oleosin 1; Prunus dulcis, gi\|460803	19.1 %	3.62 %
Oleosin A	J	18.2	YVTGEQPSGADTSDEASHR	H. annuus	Oleosin 1; Prunus dulcis, gi\|460803	19.1 %	3.62 %
Oleosin B	M^c	15.1	YDQQTHSQSTSR	gi\|266694	Oleosin H-isoform; Ficus pumila, (var. Awkeotsang) gi\|148283921	17.0 %^c	0.28 %
			QTAGSVPESLDYVK	H. annuus
			IQSTAHEMGDQGQVGVHAQVGGGK
			LQDLVEYTGQK
			RLQDLVEYTGQK
Oleosin C	K	17.0	STMSVPDQMDYVK	gi\|1345520	Oleosin 18.2 KDa; Gossypium hirsutum, gi\|167363	19.8 %	0.78 %
Oleosin C	K	17.0	LQDVGEYTGQK	H. annuus	Oleosin 18.2 KDa; Gossypium hirsutum, gi\|167363	19.8 %	0.78 %
Oleosin D	L	16.3	LQDLVEYTGQK	EL512172 H. exilis	Oleosin S1-2; Brassica napus, gi\|196122060	22.6 %	5.84 %
Oleosin D	L	16.3	AHDIGPEGAVHAGSAVGGAK	EL512172 H. exilis	Oleosin S1-2; Brassica napus, gi\|196122060	22.6 %	5.84 %
Oleosin E	M^c	15.1	YATGGHPLGSDSLDQAR	EL511252 H. exilis	Oleosin low-molecular-weight isoform; Linum usitatissimum, gi\|77737584	17.0 %^c	0.28 %
Total						100.0 %

Gel shown in Supplementary Figure 1

^aMeasure estimated from migration on SDS-PAGE

^bStandard error

^cBand contained peptides for two distinct oleosins and thus the optical density quoted is for the combination of both isoforms