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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mucosal Immunol. 2012 Jul 11;6(2):267–275. doi: 10.1038/mi.2012.67

Figure 1. Anti-CD25 Ab treatment depletes CD25+ cells, but not Foxp3+ cells, in SAMP mice.

Figure 1

(A) Representative dot plots of Foxp3 and CD25 expression on MLN CD4+ cells showing complete depletion of CD25+ cells in anti-CD25 Ab treated SAMP mice (left panel). (B) The proportion of CD4+Foxp3+ cells was significantly higher in MLN cells of SAMP versus AKR mice. The total number of CD4+Foxp3+ cells in SAMP MLN was 3 to5 times more than that observed in AKR MLN (data not shown). Following anti-CD25 Ab treatment for 6 wks, 28.3% of Foxp3+ cells in MLN of SAMP mice were eliminated, compared to 72.0% in AKR mice. (C) CD25−Foxp3+ cells significantly increased after anti-CD25 Ab treatment in MLN cells of SAMP mice, compared with AKR controls. Data are presented as the mean ± SEM (*P<0.05, **P<0.01, ***P<0.001). Control mAb SAMP, n=8; anti-CD25 Ab SAMP & control mAb AKR, n=6 each; anti-CD25 AKR, n=4.