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AC-MFB inhibits HIF-1α from hepatocellular cancer cell. Effect of various concentrations of AC-MFB on intracellular HIF-1α levels in culture of HA22T and 1MEA.7R.1 HCC cells. Cells were pretreated with 200 μM DFO for 2 h followed by AC-MFB for 2 h. All the data were normalized to the control group (AC-MFB 0 mg/mL). Data from three independent experiments in triplicate were expressed as mean ± standard error of mean; *P < 0.05 versus 200 μM DFO.
