Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 7;2013:593219. doi: 10.1155/2013/593219

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Uma Basavanna et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

(a) BoNT/A complex activity and comparison with BoNT/A holotoxin. Clone 5A3 cells were subjected to assay protocol to test the activity of BoNT/A holotoxin and complex toxin. PC12 cells were grown in 96 well plates in the presence of indicated BoNT/A holotoxin (⋄) and complex toxin (□) at 0.03, 0.05, 0.15, 0.3, 1.5, 3.0, and 10 nM concentrations. Data are presented as means ± SD (n = 3). The fluorescence measurements were performed by BioTek microplate reader. (b) BoNT/E complex activity and comparison with BoNT/E holotoxin. Clone 5A3 cells were subjected to assay protocol to test the activity of BoNT/E holotoxin and complex toxin. PC12 cells were grown in 96 well plates in the presence of indicated BoNT/E holotoxin (△) and complex toxin (□) at 0.03, 0.05, 0.15, 0.3, 1.5, 3.0, and 10 nM concentrations. Data are presented as means ± SD (n = 3). The fluorescence measurements were performed by BioTek microplate reader.