Figure 1.

The principle of the Duolink proximity ligation assay. (a) Binding of primary antibodies to PTPIP51 and the IR or PKA. (b) Binding of secondary antibodies, PLA probe PLUS and PLA probe MINUS. (c) Connector oligos only hybridize if both proteins are closer than 40 nm. (d) Ligation of the complex forms a circular template. (e) Rolling circle amplification. (f) Amplification yields a some hundredfold replication, and added fluorophore labelled probes highlight the reaction, resulting in a point-shaped signal. Figure 1 is a reproduction of the Olink instruction manual by courtsey of Olink Bioscience.