Figure 4.

Two-dimensional Western blotting analysis of anti-3-nitrotyrosine-positive proteins in a human pituitary (70 ug protein per 2D gel). (a) Silver-stained image on a 2D gel before the transfer of proteins onto a PVDF membrane. (b) Silver-stained image on a 2D gel after the transfer of proteins onto a PVDF membrane. (c) Western blot image of anti-3-nitrotyrosine-positive proteins (anti-3-nitrotyrosine antibodies + secondary antibody). (d) Negative control of a Western blot to show the cross reaction of the secondary antibody (only the secondary antibody; no anti-3-nitrotyrosine antibody). Reproduced from Zhan and Desiderio (2007) [16], with permission from Elsevier Science, copyright 2007.