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Experimental flow chart to identify nitroprotein and nitroprotein-protein complexes with NTAC-based vMALDI-LTQ MS/MS. The control experiment (without any anti-3-nitrotyrosine antibody) was performed in parallel with the NTAC-based experiments. Reproduced from Zhan and Desiderio (2006) [4], with permission from Elsevier Science, copyright 2006.
