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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Cell Signal. 2012 Oct 17;25(1):359–371. doi: 10.1016/j.cellsig.2012.10.009

Fig. 11. CIKS expression is enhanced in aortas of streptozotocin (STZ)-induced type 1 diabetic mice.

Fig. 11

A, Blood glucose levels are increased following STZ administration. Male C57Bl/6 mice were administered once daily for 4 days with STZ in sodium citrate buffer (60 mg/kg, IP). The control group received sodium citrate buffer alone. Blood glucose levels were quantified at 3, 5, 10, and 17 days post-STZ. *P < at least 0.05 vs. control (n=4/group). B, DPI-inhibitable ROS is increased in aortas of STZ-induced type 1 diabetic mice. ROS production was measured by the lucigenin-enhanced chemiluminescence assay using aortic homogenates from the indicated groups. The reaction mixture contained 100 μM NADPH. Experiments were also performed in the presence of DPI (10 μM in DMSO). *P < 0.05 vs. saline (n=4/group). C, CIKS protein expression is enhanced in aortas of STZ-treated mice. Aortas from mice described in A were analyzed for CIKS expression by immunoblotting. Densitometric analysis of the immuno-reactive bands is summarized in the lower panel. *P < 0.05 vs. control (n=3/group). D, CIKS, adhesion molecule and IL-6 mRNA expression are increased in aortas from STZ-treated mice. Aortas from mice described in A were analyzed for CIKS, ICAM-1, VCAM-1 and IL-6 mRNA by RT-qPCR. *P < at least 0.05 vs. control (n=4/group).