. A, B, HG induces ICAM-1 expression. At 70% confluency, the complete medium on HAEC was replaced with EBM-2 (without supplements) for 2h, incubated with HG for the indicated time periods, and analyzed for ICAM-1 protein by immunoblotting (A; n=3) and mRNA expression by RT-qPCR (B). B, *P < at least 0.05 vs. NG. C, D, HG-induced ICAM-1 expression is CIKS dependent. HAEC infected with lentiviral particles expressing CIKS shRNA (MOI 0.5 for 48 h) were treated with HG and analyzed for ICAM-1 protein (C; n=3) and mRNA (12 h; D) expression as in A and B. D, *P < at least 0.01 vs. NG, P < 0.01 vs. HG + copGFP (n=6). E, F, HG induces VCAM-1 expression. HAEC incubated with HG as in A and B were analyzed for VCAM-1 protein (E; n=3) and mRNA expression (F). F, *P < at least 0.05 vs. NG (n=6). G, H, HG-induced VCAM-1 expression is CIKS dependent. HAEC treated as in C and D were analyzed for VCAM-1 protein (G; n=3) and mRNA (12 h; H) expression. H, *P < at least 0.01 vs. NG, P < 0.01 vs. HG + copGFP (n=6).